ProMark: How it Works
ProMark’s automated proteomics imaging platform works on standard formalin-fixed, paraffin-embedded tissue sections mounted on slides.
After antigen retrieval, the tissue sections are subjected to multiplex immunofluorescent staining with monoclonal antibodies as well as DAPI using a proprietary assay format that enables the quantitative biomarker measurements in the "region of interest" on the tissue sections. The "raw" images of the stained sections are acquired on our image acquisition platform and then transferred to our advanced imaging platform, which through advanced object recognition and usage of our proprietary script enables digitalized quantitative measurements in the defined tissue sub-regions.
Metamark’s CLIA-certified, CAP-accredited laboratory, located in Cambridge MA, typically receives sections already cut and mounted on slides, but can receive paraffin tissue blocks, as well. The entire process of slide staining, quantitative biomarker protein measurements in the defined tissue regions, and MPRS result for each patient is fully automated, driven by the bar-code integrated with our Laboratory Information System.